Antibodies to filamentous actin (F-actin) in type 1 autoimmune hepatitis
نویسندگان
چکیده
منابع مشابه
Antibodies to actin in autoimmune haemolytic anaemia
BACKGROUND In autoimmune haemolytic anaemia (AIHA), autoreactive antibodies directed against red blood cells are up-regulated, leading to erythrocyte death. Mycoplasma suis infections in pigs induce AIHA of both the warm and cold types. The aim of this study was to identify the target autoantigens of warm autoreactive IgG antibodies. Sera from experimentally M. suis-infected pigs were screened ...
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In an effort to characterize the cellular antigens recognized by anti-neutrophil antibodies in autoimmune neutropenia, we studied sera, purified immunoglobulin G (IgG) and isolated F(ab')2 from 70 neutropenic patients suspected of this diagnosis. Anti-neutrophil antibodies were found in the sera of 36 of these patients by either 125I-staph A binding or immunofluorescence cytometric techniques t...
متن کاملQuantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons.
Filamentous actin protein (F-actin) plays a major role in spinogenesis, synaptic plasticity, and synaptic stability. Changes in dendritic F-actin rich structures suggest alterations in synaptic integrity and connectivity. Here we provide a detailed protocol for culturing primary rat cortical neurons, Phalloidin staining for F-actin puncta, and subsequent quantification techniques. First, the fr...
متن کاملAdenosine Triphosphate Cleavage during the G-actin to F-actin Transformation and the Binding of Adenosine Diphosphate to F-actin.
Since the discovery by Straub and Feuer (1) and by Laki, Bowen, and Clark (2) that adenosine triphosphate bound to Gactin is transformed to adenosine diphosphate and inorganic phosphate during polymerization of actin, it has become increasingly clear that the chemical changes in the nucleotide are related to the change in the physical state of the protein. Barany, 13iro, Molnar, and Straub have...
متن کاملMobility of filamentous actin in living cytoplasm
Filamentous actin in living cultured cells was labeled by microinjecting trace amounts of rhodamine-phalloidin (rh-pha) as a specific, high-affinity probe. The microinjection caused no detectable effect on cell morphology or cell division. The distribution of rh-pha-labeled filaments was then examined in dividing cells using image-intensified fluorescence microscopy, and the exchangeability of ...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 2006
ISSN: 0021-9746
DOI: 10.1136/jcp.2005.027367